The draw for the round of 32 of the 6th edition of the FUFA Women’s Cup will be conducted on Wednesday 26th February 2025 at FUFA House, starting at 10:00 am and will be live on FUFA Tv.
The draw will feature 32 teams, which will be divided into two pots.
Pot A: Will have the 12 Finance Trust Bank FUFA Women Super League Teams and the top two teams from each of the Finance Trust Bank FUFA Women Elite League Groups (4 teams).
Pot B: Will have the remaining 10 Finance Trust Bank FUFA Women Elite League teams and 6 regional teams.
| S/N | Pot A | Pot B |
| 1 | Kawempe Muslim Ladies FC | She Geme FC |
| 2 | Kampala Queens FC | Lango Queens FC |
| 3 | Uganda Martyrs Lubaga | Pabo United FC |
| 4 | She Corporate FC | UCU Lady Cardinals FC |
| 5 | Makerere University WFC | Goldstar Ladies FC |
| 6 | Amus College | Isra Soccer Academy WFC |
| 7 | Lady Doves FC | Sacred Hearts WFC |
| 8 | She Maroons FC | Royal Dreamers WFC |
| 9 | Wakiso Hill WFC | Ateker WFC |
| 10 | Rines SS WFC | She Kasese FC |
| 11 | Olila H/S WFC | Uganda Martyrs Lubaga Juinor |
| 12 | FC Tooro Queens | Busoga Queens FC |
| 13 | St. Noa Girls FC | Jinja City Queens FC |
| 14 | Kawempe Muslim SS | SIA International Academy |
| 15 | Asubo Ladies FC | Boni Consilii Girls FC |
| 16 | Highway Ladies FC | Lady Eagles FC |
